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  • Rapid detection of E. coli cells in urine samples using a ...

    Escherichia coli is one of the main causes of urinary tract infections (UTIs). E. coli is commonly detected from urine using standard culture method. However, the urine sampling and analysis required for these methods can be costly, time consuming (requires 24 to 48 hours) and labor-intensive. This …

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  • A multiplex PCR method to detect 14 Escherichia coli ...

    E. coli strains belonging to 14 serogroups, including O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O25, O75 and O83, are the most frequently detected UPEC strains in a diverse range of clinical urine specimens. In the current study, the O-antigen gene clusters of E. coli serogroups O1, O2, O18 and O75 were characterized.

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  • Urine Culture with E. Coli: Meaning, Results and Treatment ...

    2021-7-11 · The presence of E. coli in urine usually indicates a urinary tract infection, but it may not always cause visible symptoms. When symptoms appear, they may include a burning sensation while urinating, a strong urge to urinate, passing small amounts of urine, passing strong-smelling urine, and urine that appears cloudy or red.

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  • Rapid and sensitive detection of fluoroquinolone-resistant ...

    2007-10-15 · Although this assay was designed to detect fluoroquinolone-resistant E. coli in urine, it is principally possible to use it for other clinical specimens like blood or secretion. However, for an application of this assay in clinical routine diagnostics further development is required in order to increase the discrimination capabilities in mixture experiments.

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  • Impedimetric method for measuring ultra-low E. coli ...

    2015-4-15 · In this study, we developed an interdigitated gold microelectrode-based impedance sensor to detect Escherichia coli (E. coli) in human urine samples for urinary tract infection (UTI) diagnosis.E. coli growth in human urine samples was successfully monitored during a 12-h culture, and the results showed that the maximum relative changes could be measured at 10 Hz.

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  • Impedimetric method for measuring ultra-low E. coli ...

    A linear relationship between the impedance change and initial E. coli concentration was obtained with the coefficient of determination R (2)>0.90 at various growth times of 1, 3, 5, 7, 9 and 12h in urine. Thus our sensor is capable of detecting a wide range of E. coli concentration, 7×10 (0) to 7×10 (8) cells/ml, in urine samples with high ...

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  • Microfluidic smartphone quantitation of Escherichia coli ...

    2019-12-1 · In this study we present a sensitive and rapid optical sandwich fluorescent immunoassay able to detect and quantify E. coli K12 in less than 25 min in synthetic urine without the need of sample preparation, with the assistance of a smartphone camera. The new sandwich immunossay presents several advantages, such as: 1) affordable off-the-shelf immunoassay …

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  • Multiplex PCR Based Urinary Tract Infection (UTI) Analysis ...

    2020-2-1 · In 88 patients (15%, 88/582), both PCR and urine culture identified the same pathogens and the same number of bacteria. In 85/88 cases (97%) both culture and PCR reported a single pathogen. The most common bacteria with total agreement were E. coli (35%, 30/85), Enterococcus faecalis (19%, 16/85), and Klebsiella pneumoniae (14%, 12/85). Together, these 3 bacteria …

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  • The application of chromogenic media in clinical ...

    2007-7-30 · In a large study using 9247 urine samples, the authors reported that 94% of E. coli strains could be identified by their coloured appearance alone and no E. coli strains were misidentified. When compared with the use of conventional media (Blood and MacConkey agars), the authors reported a cost reduction of 46% for media, and a 67% reduction in the time required …

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  • Application of smartphone-based spectroscopy to

    2021-1-15 · The fluorescence signal was generated from Au nanoclusters. When AuNPs were added, the resonance transfer of fluorescent energy between the nanoclusters and E. coli occurred. Antibodies of E. coli were modified on the surface of AuNPs to capture O157: H7 in the samples. Therefore, when different concentrations of O157: H7 were added to the samples, the degree of …

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  • Quantitative detection of Escherichia coli from urine of ...

    2012-12-1 · Quantification of E. Coli in Urine Specimens and Comparison of Results. We quantified the copy numbers of E. coli from urine specimens using the standard curve described above. In all the urine samples in which E. coli was detected, we detected 5.9 × 10 7 to 1.2 × 10 2 copies/mL of E. coli.

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  • Culture-free bacterial detection and identification from ...

    2018-2-21 · A comparison of AST with chloramphenicol ... blood and using the detection electrodes prepared to detect E. coli to monitor the 2-hour ... Antimicrobial Susceptibility Testing in Urine.

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  • Impedimetric method for measuring ultra-low E. coli ...

    2021-4-27 · Fast detection of E. coli with a novel fluorescent biosensor based on a FRET system between UCNPs and [email protected] in urine Analytical Methods ( IF 2.896) Pub Date : 2021-4-6, DOI: Yuan Yao, Guoming Xie, Xin Zhang, Jinshan Yuan, Yulei Hou, Hui Chen

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  • Development of a fluorescent distance-based paper device ...

    2014-11-20 · In this study, we developed an interdigitated gold microelectrode-based impedance sensor to detect Escherichia coli (E. coli) in human urine samples for urinary tract infection (UTI) diagnosis. E. coli growth in human urine samples was successfully monitored during a 12-h culture, and the results showed that the maximum relative changes could ...

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  • Evaluation and comparison of recombinase polymerase ...

    2020-10-1 · The highly sensitive and selective determination of Escherichia coli (E. coli) in urine was achieved using a SYBR™ safe loop-mediated isothermal amplification (LAMP) method with a distance-based paper device.New primers set specific to multi-copy the 16s rRNA gene of E. coli were designed and used in this study. The detection sensitivity of these primers was higher than …

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  • DipTest: A litmus test for E. coli detection in water

    2021-1-21 · As low as 100 ag and 1 fg DNA, 4–5 CFU/mL and 101 CFU/mL of E. coli O157:H7 was detected using the stx and rfbE gene targets respectively with …

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  • A Cheaper, Faster Test to Detect E. coli in Drinking Water ...

    2017-9-6 · More » In the present work, for the first time, we have developed a simple and low-cost novel paper strip, similar to litmus paper, that can detect E.coli bacteria in water samples. The paper strip is made of a Grade GB003, Whatman absorbing gel blotting paper with one edge of the strip coated with wax hydrophobic barrier and the opposite edge (attraction zone) coated with D …

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  • Quick identification and quantification of Proteus ...

    2017-9-13 · Glacierclean, which already sells mobile water kits to detect E. coli for about 5 a test, hopes to have its DipTest strips on the market within nine months. “Simple ideas create paradigm shifts in technology and this is a simple, frugal innovation,” said Mitra, also a mechanical and mechatronics engineering professor at Waterloo.

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  • Reduction in energy for electrochemical disinfection of E ...

    2012-8-19 · Proteus mirabilis is an opportunistic pathogen that can cause urinary tract infection in human beings. The accurate and rapid identification and quantification of P. mirabilis is necessary for early treatment. In this study, a pair of specific primers according to the conserved ureR sequence of P. mirabilis was designed and novel systems which consisted of a polymerase chain …

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  • Multisite Prospective Comparison of Multiplex Polymerase ...

    Multisite Prospective Comparison of Multiplex Polymerase Chain Reaction Testing with Urine Culture for Diagnosis of Urinary Tract Infections in Symptomatic Patients ... [5, 6]. Accumulating evidence has established that Standard Urine Culture (SUC) may not detect clinically significant bacteria [7]. ... E. coli was the most detected bacterium ...

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  • A comparison of the performance of commercially available ...

    2020-9-9 · UTI medium and CPS ID2 performed similarly, each showing advantages and disadvantages in terms of sensitivity and PPV. Using E coli as an indicator, UTI medium gave a PPV of 97.8% compared with 99.4% for CPS ID2. This was the result of more pink colonies being E coli on CPS ID2 media than on UTI medium.

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  • Escherichia coli as a Model Organism and Its Application ...

    2020-6-4 · E. coli is a genetically diverse species that spans at least six well-characterized different phylogenetic groups and nine different pathotypes 40,41.Amongst these, UPEC …

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  • Invasion of vaginal epithelial cells by uropathogenic ...

    2017-9-22 · The immobilized rV5 phage-based assay was able to detect E. coli O157:H7 at 10 CFU/mL in spinach homogenate and the AG2A phage-based assay was able to detect E. coli O45:H2 at 10 CFU/mL in ground beef homogenate using both plaque assay and the qPCR technique to detect the progeny phages (Tables 8 and 9).

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  • Print to detect: a rapid and ultrasensitive phage-based ...

    2020-8-20 · Several studies have also shown that methylation CpG sites in urine can be promising markers to detect or monitor BCa (14 – 16). In a multicenter study, Bladder EpiCheck, a commercial application in Europe, used a panel of 15 methylation markers to monitor recurrence in patients with NMIBC undergoing surveillance, and its overall sensitivity ...

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  • JCI - Urine DNA methylation assay enables early detection ...

    2012-9-25 · The occurrence of microorganisms in water due to contamination is a health risk and control thereof is a necessity. Conventional detection methods may be misleading and do not provide rapid results allowing for immediate action. The quantitative polymerase chain reaction (qPCR) method has proven to be an effective tool to detect and quantify microorganisms in …

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  • Application of quantitative PCR for the detection of ...

    2014-4-7 · Common specimens include urine, faeces, wound swabs, throat swabs, vaginal swabs, sputum, and blood. Less common, but important specimens include cerebrospinal fluid, pleural fluid, joint aspirates, tissue, bone and prosthetic material (e.g. line tips). Some types of specimen are normally sterile e.g. blood, CSF. These samples are

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  • 10 METHODS OF ISOLATION OF - National Institute of Open ...

    Non-pathogenic of E. coli K12, Staphylococcus epidermidis (S. epidermidis) and Bacillus subtilis (B. subtilis) were also included in this study to evaluate the selectivity of the biosensor. As observed in previous studies [92,94,97], magainin I showed selectivity for pathogenic E. coli.

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  • Molecules | Free Full-Text | Antimicrobial Peptides ...

    2020-4-21 · Nucleic acid is the main material for storing, copying, and transmitting genetic information. Gene sequencing is of great significance in DNA damage research, gene therapy, mutation analysis, bacterial infection, drug development, and clinical diagnosis. Gene detection has a wide range of applications, such as environmental, biomedical, pharmaceutical, agriculture and …

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  • Urinary tract infection: detection of Escherichia coli ...

    The hyperimmune serum reacted with all urine specimens studied. However, bands of 77, 66, 51, 43, and 21 kDa were detected in urine specimens positive for E. coli. The urine specimens that were positive for heterologous bacteria showed one to four bands, which were combination of bands 77, 66, 51, 24, and 22 kDa.

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  • Determining the best method to detect E. coli - StarFish ...

    2021-3-15 · What does it mean to have E coli in your urine? Infection of the bladder (cystitis).. This type of UTI is usually caused by Escherichia coli (E.coli), a type of bacteria commonly found in the gastrointestinal (GI) tract. However, sometimes other bacteria are responsible. Sexual intercourse may lead to cystitis, but you don’t have to be sexually active to develop it.

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  • Often asked: How can you get e coli in your urine?

    2017-9-25 · Travel up urethra: E. Coli species are the most common bowel bacteria and are invariably present on the perineal skin, no matter how well you wipe. They will be present in any urine temporarily trapped behind the labia and get 'sucked' up the urethra during terminal muscle contractions at end of voiding. E. Coli around the urethral opening can be pushed up the urethra …

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  • how do you get e-coli in your urine? | Answers from ...

    What we claim is: 1. A method of detecting the presence of E. coli and/or other coliforms in a sample of interest comprising the steps of: (1) preparing a medium containing an indicator which is a galactoside or a glucuronide, and Cefsulodin, (2) applying a portion of said sample to the medium prepared in step (1), (3) incubating the product of step (2) for a time sufficient to cause evidence ...

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  • First Instant Test to Detect Presence of E. Coli 1057:H7

    The test can be used in a diagnostic or research laboratory to accurately detect E. coli and can help with quality control in cattle facilities. 'The novelty of this test is that it targets four genes,' Nagaraja said. 'We are constantly working on finding better and more sensitive ways to detect these pathogens of E. coli in cattle feces.' To ...

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  • Method for detection for total coliforms and E. coli - The ...

    What is this bacteria in the urine? The Escherichia coli or E. coli , a bacteria normally found in the intestines of humans and animals. It is responsible for more than 85 percent of all urinary tract infections. Not only is there one type of E. Coli, and there is a wide variety of infections and negative symptoms that can occur when this bacteria becomes a problem.

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  • Researchers Create Better Methods to Detect E. coli | Lab ...

    2021-7-8 · Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) has so far infected almost a hundred of millions of people and caused more than a million of death across the world. Many serological tests have been developed to track down virus infection in community via identification of antibodies against SARS-CoV2 virus. However, the tests vary in sensitivity, …

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  • Separating and Detecting Escherichia Coli in a ...

    2011-8-12 · detect E. coli [33]. Some have been implemented in a miniaturized form of detector [33]. These IMS/IS techniques presented focus on detecting E. coli O157, which are a cause of foodborne illness [15]. Wright et al. used IMS to collect and detect E. coli …

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  • E. coli and Total Coliform Detection in Drinking Water

    2015-8-1 · E. coli . results . compared to the Colilert ®-18 reference method for possible verification in Denmark. The verified detection technologies utilize fluorogenic enzyme substrate compounds to detect the presence of enzymes specific to TC and . E. coli. Environmental and Public Health Regulatory Background of Total Coliform and . E. coli . at a ...

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  • Escherichia coli Global Gene Expression in Urine from ...

    2010-11-11 · Of these ten E. coli-positive samples, two urine specimens contained mixed infections of two different E. coli strains. O and H serotyping conducted on these 12 E. coli isolates indicated that O6 and O25, which are frequently associated with UTI isolates [37] , were the most common serogroups, representing 7 of 12 strains ( Table S1 ).

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  • Method for detection for total coliforms and E. coli - The ...

    What we claim is: 1. A method of detecting the presence of E. coli and/or other coliforms in a sample of interest comprising the steps of: (1) preparing a medium containing an indicator which is a galactoside or a glucuronide, and Cefsulodin, (2) applying a portion of said sample to the medium prepared in step (1), (3) incubating the product of step (2) for a time sufficient to cause evidence ...

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  • Rapid test strips detect bacterial contamination in ...

    2012-4-30 · Researchers have developed a rapid testing method using a simple paper strip that can detect E. coli in recreational water within minutes. The …

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  • Detection of blaNDM-1 gene in ESBL producing Escherichia ...

    2021-4-30 · This study aimed to detect carbapenem-resistant blaNDM-1 gene in ESBL producing E. coli and K. pneumoniae isolates. Methodology A total of 190 E. coli and 350 K. pneumoniae isolates were screened for extended spectrumβ-lactamase (ESBL), carbapenemase and metallo β-lactamase (MBL) production via double-disk synergy test (DDST), modified Hodge ...

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  • what cause e coli in urine | Answers from Doctors | HealthTap

    2021-2-2 · e.coli in urine culture means it is in your bladder? what uti or diseases can a person get if e.coli has been diagnosed in urine culture? Dr. Douglas Miller …

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  • Smartphone sensor can detect dirty water

    Existing methods to detect E.coli are slow and often expensive, so we set out to develop a faster, cheaper approach using a small biosensor operated from your smartphone. Finding faeces in drinking water. Scientifically speaking, E. coli is a normal flora in the mammal intestinal track. So when we find it in a water body, such as a lake or a ...

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  • A sensitive gold-nanorods-based nanobiosensor for

    2019-3-26 · To evaluate whether the designed nanobiosensor and real-time PCR can simultaneously detect both C. jejuni and C. coli species in stool specimens, an alternative duplex-PCR assay was also done on the specimens as previously described . C. jejuni ATCC 29428 and C. coli ATCC 43478 strains were used as reference strains in the method.

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  • Identification of Pathogens by Mass Spectrometry ...

    2010-4-1 · CE-MS is a useful technology for the discovery of biomarkers from pathogens ( 28 ). Hu et al. ( 29) identified bacterial species-specific proteins in a sample by performing CE-MS analysis on the proteolytic digest of the cell extract and monitoring …

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